12th Annual Symposium
Physics of Cancer
Leipzig, Germany
Aug 30 - Sept 1, 2021
Contributed Talk
Innovative tools and surfaces for 3D cell culture and single cell arrays
Elias Horn
ibidi GmbH, Lochhamer Schlag 11, 82166 Gräfelfing, Germany
Contact:  | Website
The investigation of cancer in vitro is constantly moving from the classical 2D cell monolayers towards 3D tumor models with both, higher complexity and relevance. Using specialized micro-fluidic geometries, cell cultureware can be created in order to cultivate 3D tumor models, organoids and tissue under physiologically relevant conditions. These tools can contribute to better mimic the cells´ in vivo environment and optimize the results in the scientific experiment.

Not only the culture conditions have evolved, but also the analysis. Instead of averaging the results of an entire cell population the behavior of each single cell is moving into the focus. Micro-patterned surfaces are powerful tools for creating single cell arrays. As an example, we developed a method which allows for high throughput, fluorescent probe independent CAR-T cell potency evaluation on a single cell level. Therefore, we created an assay which facilitates identification and tracking of CAR-T and target cancer cells with machine learning based image analysis. Using our approach, we were able to analyze and track interaction and killing events, viability status and apoptotic body formation with high throughput and using just phase contrast imaging.
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