12th Annual Symposium
Physics of Cancer
Leipzig, Germany
Aug 30 - Sept 1, 2021
Invited Talk
Probing and manipulating cell signalling pathways with magnetogenetics
Andreas Neusch1, Daniel Kuckla1, Juliia Novoselova1, Koceila Aizel2, Elie Balloul2, Chiara Vicario2, Domenik Lisse2,3, Jean-Michel Siaugue4, Christine Ménager4, Jacob Piehler3, Matthieu Coppey2, Maxime Dahan2, Cornelia Monzel1,2
1Heinrich-Heine University Düsseldorf, Germany
2Institut Curie, Paris, France
3Osnabrück University, Germany
4Université Pierre et Marie Curie, Paris, France
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Many cell functions rely on the coordinated activity of signalling pathways on subcellular
scales. However, there are few tools capable of probing and perturbing signalling networks
remotely and with a spatial resolution matching the intracellular dimensions of the networks
activity pattern. Here, we develop a generic magnetogenetic approach where functionalized
magnetic nanoparticles (MNPs) target an intracellular protein of interest to trigger cell signaling
functions. These MNP-protein complexes act as nanoscopic hot spots that can be displaced by
magnetic forces at distance to provide molecularly graded information to the cell and to trigger
a signal transduction pathway. We bio-orthogonally functionalize these MNP for capturing of
intracellular proteins and demonstrate the manipulation of Rho-GTPases - a set of molecular
switches known to regulate cell migration via complex spatiotemporal patterns of activity. We
then present prospective applications of Magnetogenetics in cancer progression and
regenerative medicine.
[1]I. Novoselova*, A. Neusch*, J.-S. Brand, M. Otten, M.R. Safari, M. Karg, M. Farle, U. Wiedwald and C. MonzelMagnetic Nanoprobes for Spatio-Mechanical Manipulation in Single Cells, *equal contribution (submitted) (2021)
[2]L. Toraille, K. Aïzel, E. Balloul, C. Vicario, C. Monzel, M. Coppey, E. Secret, J.-M. Siaugue, J. Sampaio, S. Rohart, N. Vernier, L. Bonnemay, T. Debuisschert, L. Rondin, J.-F. Roch and M. Dahan,Optical magnetometry of single biocompatible micromagnets for quantitative magnetogenetic and magnetomechanical assays, Nano Letters (2018), 18, 7635-41 (2018)
[3]D. Lisse*, C. Monzel*, C. Vicario, M. Coppey, J. Piehler and M. DahanEngineered Ferritin for Magnetogenetic Control of Proteins and Organelles Inside Living Cells, dvanced Materials (2017), 29, 1700189 *equal contribution (2017)
[4]C. Monzel, C. Vicario, M. Coppey and M. DahanMagnetic Control of Cellular Processes Using Biofunctional Nanoparticles, Chemical Science (2017), 8, 7330-8 (2017)
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