9th Annual Symposium
Physics of Cancer
September 24-26, 2018
|PoC - Physics of Cancer - Annual Symposium|
Focal adhesion - hemidesmosome crosstalk in migrating keratinocytes
Institute of Molecular and Cellular Anatomy, RWTH Aachen University, 52074 Aachen
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Keratin-anchoring hemidesmosomal cell-matrix anchoring sites contribute to the mechanical integrity of epithelia. Their role in epithelial cell migration, however, remains unclear. We find that hemidesmosomes cluster in migrating human primary keratinocytes as arrays consisting of multiple chevrons that are flanked by actin-associated focal adhesions. The arrays extend from the cell rear to the cell front. New hemidesmosomal chevrons form subsequent to focal adhesion assembly at the cell's leading front whereas chevrons and associated focal adhesions disassemble at the cell rear. The bulk of the hemidesmosome-focal adhesion composite, however, remains in place during cell translocation. Similar hemidesmosome-focal adhesion patterns emerge during substrate adhesion and cell spreading. We further find that hemidesmosomes and focal adhesions affect each other's distribution. Our results provide evidence that both junctions are separate but linked entities that treadmill coordinately to support efficient directed cell migration. They further suggest that both junctions cooperate to coordinate the dynamic interplay between the keratin and actin cytoskeleton.