9th Annual Symposium
Physics of Cancer
September 24-26, 2018
|PoC - Physics of Cancer - Annual Symposium|
Polarized dynamics of intermediate filament in glial cell migration
Institut Pasteur Paris CNRS UMR3691, Cell Polarity, Migration and Cancer Unit, 25 rue du Dr Roux 75724, Paris Cedex 15, France
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Intermediate filaments (IFs) are key players in the control of cell morphology and structure as well as in active processes such as cell polarization, migration and mechano-responses. However the regulatory mechanisms controlling IF dynamics and organization in motile cells are still poorly understood. Using a combination of cutting edge quantitative microscopy techniques and theoretical analysis, we investigated the mechanisms leading to the polarized rearrangement of the IF network along the polarity axis during glial cell migration. We show that the spatial distribution of the cytoplasmic IFs results from a continuous turnover based on the cooperation of an actin-dependent retrograde flow and anterograde and retrograde microtubule-dependent transports. During wound-induced polarization, IF transport becomes directionally biased from the cell center towards the cell front. Such asymmetry in the transport is mainly caused by a Cdc42- and aPKC-dependent inhibition of dynein-dependent retrograde transport. Our results show how polarity signaling can affect the dynamic turnover of the IF network to promote the polarization of the network itself.