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| Contributed Talk, Friday, 10:00 – 10:15 |
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| Dynamics of Growing Cell Populations
Anna-Kristina Marel, Joachim O.
Rädler
Ludwig-Maximilians-Universität
München, Faculty of Physics and Center for NanoScience (CeNS), Soft
Condensed Matter Group, Geschwister-Scholl-Platz 1, 80539 Munich, Germany |
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Dynamic remodelling of tissue involves cell deformation, cell migration,
cell division and cell death. Here we study the migration of cells from
defined microscopic geometries. We prepared reproducible three-dimensional
micro-structured array made of PEG-DMA. The structures are filled with
cells and serve as equal sources of several migration experiments in parallel.
Using image processing we access velocity, growth rate and cell density
over time. Due to the confinement, the initial small cell colonies show
a higher cell density than freely grown cell groups that have not yet reached
confluency. We observe that the cell density relaxes over a time scales
corresponding to the doubling time of the cell line. The area of the micro-colony
increases more strongly during the initial migration process and crosses
then over to exponential growth due to cell division. We analyze the evolution
of shape and polarisation of the patches as well as the distinct center-of-mass
movement.
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