8th Annual Symposium
Physics of Cancer
October 4-6, 2017
|PoC - Physics of Cancer - Annual Symposium|
Disrupting Chemotaxis using Contact Guidance of Nanotopography
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Cell migration is important in many processes such as wound healing and cancer metastasis. The physical features of the surface over which cells move affect migration outcomes. In this context, we aim to examine the effect of ridged surface textures on collective cellular migration. The well-studied social amoeba Dictyostelium discoideum (Dicty) is a model system with a well-defined developmental cycle. When starved, Dicty cells follow each other and migrate together in streams to form clumps of cells called mounds. Cyclic AMP signaling facilitates this migration, resulting in directional polymerization of the cytoskeletal protein actin.
The textured surface causes a distinct phenotype change when compared to the control: Initially the cells are more active, however, the cells are not able to enter the streaming part of their developmental cycle. We demonstrate that the competition between the directional cues from the surface and the chemical cues from the autocrine cAMP signaling is sufficient to disrupt the development cycle of the amoeba.