8th Annual Symposium
Physics of Cancer
October 4-6, 2017
|PoC - Physics of Cancer - Annual Symposium|
The weakness of senescent fibroblasts
University of Ulm, Institute for Experimental Physics, 89069 Ulm
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Cellular senescence and cancer are to sides of one coin. Senescence can be thought of as a mechanism to prevent aged cells going bad. However, while lots of efforts have been committed to characterize the properties of cancer cells, the characterization of the biophysical manifestation of cellular senescence is still in its infancy. Here, we thoroughly characterize the biophysical state of senescent dermal fibroblasts using a combination of single-cell techniques. Our results demonstrate that changes in composition of the cytoskeleton together with loss of myosin-II activity lead to a decrease in the elasticity and tension of senescent fibroblasts cytoskeleton. This is accompanied by nuclear deformations, a significant loss of traction forces, and a decrease in the wound healing capacity. Taken together, we show that the ability of senescent dermal fibroblasts to generate intra- or extracellular tension is severely attenuated. A lack of tension has deleterious effects for building the extracellular matrix, for wound healing, and may lead to epigenetic changes involved in senescence.