7th Annual Symposium
Physics of Cancer
October 4-6, 2016
|PoC - Physics of Cancer - Annual Symposium|
Cell Binding Peptides from Statistical Analysis of Random Peptide Phage Display Libraries
Fraunhofer Institute for Cell Therapy and Immunology (IZI), Perlickstraße 1, 04103 Leipzig, Germany
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An innovative, highly diverse (>109) peptide phage library has been generated with a novel tri-nucleotide synthesis approach, with less than 20 codons per position, perfectly statistically distributed and a peptide diversity outnumbering most any present library. This diversity, in combination with next generation sequencing and novel software allows for the first time that phage display results are interpreted on a completely statistical basis in silico. For example it allows determining epitopes after single round panning directly on complete sera from allergy patients or on polyclonal antibodies. In collaboration with CNUHH we started looking at peptides binding to general tumor tissues. In another project together with partners from the pharmaceutical industry we have identified kidney tissue specific peptides. These approaches have led to several insights into how statistical search for binding motifs in large data sets can be accomplished. We have now several peptides in the pipeline for further development and multiple applications.