7th Annual Symposium
Physics of Cancer
Leipzig, Germany
October 4-6, 2016
Invited Talk
Micro-Structured Surfaces for Assessment of Migratory Phenotypes
J. O. Rädler
Ludwig-Maximilians-University Munich, Geschwister-Scholl-Platz 1, 80539 München, Germany
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Collective cell migration is a hallmark in cancer development and progression. One of the challenges in the field is to bridge collective cell dynamics to single cell migration. The talk provides a perspective on how micro-structured surfaces, which confine cell migration, facilitate quantitative assessment of migratory phenotypes. Using time-lapse microscopy we first analyze the directed flow of Madin–Darby canine kidney (MDCK) cells in micro-channels and examine velocity correlations and spontaneous formation of vortices. We compare the collective behavior with the distinct modes of rotational migration found in confined mesoscopic systems consisting of a small number of cells on circular micro-pattern. The particular vortex states are reproduced in computer simulations based on a generalized Potts model. In agreement with experiment the model shows that vortex stability depends on the interplay of the spatial arrangement and internal polarization of neighboring cells. We then report on novel modes of migration observed in ring-shaped micro-lanes with artificial barriers and dumbbell-like structures. We demonstrate that micro-patterned surfaces open up novel approaches to probe multiple motility parameters with potential applications in cancer research and drug screening.
[1]A. K. Marel, M. Zorn, C. Klingner, R. Wedlich-Soldner, E. Frey and J.O. Rädler, Biophysical Journal 107(5), 1054 (2014)
[2]F. J. Segerer, F. Thüroff, A. P. Alberola, E. Frey, and J.O. Rädler, Physical Review Letters 114, no 22 228102 (2015)
[3]C. Schreiber, F. J. Segerer, E. Wagner, A. Roidl, and J. O. Rädler, Scientific Reports 6 26858 (2016)
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