7th Annual Symposium
Physics of Cancer
October 4-6, 2016
|PoC - Physics of Cancer - Annual Symposium|
Membrane-Targeting DNA Nanostructures
Molecular Biophysics group, Institute of Experimental physics I, Universität Leipzig, Linnéstr. 5, 04103 Leipzig, Germany
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Almost one third of the genes in most genomes encode for membrane proteins. Sitting at the interface between cell interior and exterior, these types of molecules are important drug targets. One approach in order to understand the mechanisms of membrane proteins is to build artificial mimics that reconstitute certain functionalities of their natural counterparts. Recent developments in DNA nanotechnology provide now a powerful toolbox too build multifunctional and complexly shaped macromolecules with atomic precision. Here we show how DNA nanostructures assembled by the origami technique can be attached to lipid membranes and how functional membrane protein mimics can be obtained. Examples include large scale membrane deformations in analogy to coat-forming proteins, e.g. from the I-/F-BAR family, as well as DNA-based nanopores. Furthermore, fundamental properties of the DNA nanostructure lipid membrane interaction, such as the two-dimensional diffusive motion and the domain localization of these structures are characterized. Finally we show how DNA nanostructures can be integrated into carrier systems assembled by the layer-by-layer method providing a new approach for the assembly of multi-compartment drug delivery systems.