7th Annual Symposium
Physics of Cancer
October 4-6, 2016
|PoC - Physics of Cancer - Annual Symposium|
Death by Gold: Targeting Invasive Glioblastoma Cells by Peptide-Functionalized Gold Nanorods
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Cancer stem cells (CSCs) are known to be responsible for drug resistance and tumor recurrence in several cancer types, making their eradication a primary objective in cancer therapy. Glioblastoma multiforme (GBM) tumors are usually composed of a highly infiltrating CSC subpopulation, which has Nestin as a putative marker. Since the majority of these infiltrating cells are able to elude conventional therapies, we have developed a gold nanorod- (AuNR) peptide-biosensor capable of specific recognition and selective eradication of Nestin-positive GBM (GBM-Nes+) infiltrating cells, by photohermolysis. The AuNR-peptide biosensor was evaluated regarding heating abilities, cell selectivity, cell uptake pathway, and photohthermal activity in mono- and co-culture systems containing Nes+ and Nestin-negative (Nes-) GBM cells. Biodegradable three dimensional (3D) hydrogels, composed of star shaped poly(ethyleneglycol) (starPEG) covalently connected to matrix metalloproteinase-susceptible peptides and heparin (starPEG-MMP-heparin), were used to recreate GBM tumor microenvironments, and for the assessment of AuNR photothermal activity. Finally, the obtained 3D culture results were then compared with two dimentional monolayer cultures.