6th Annual Symposium
Physics of Cancer
Leipzig, Germany
September 7-9, 2015
Contributed Talk
Dynamics of cancer tissue
Ann-Katrine West1, Lena Wullkopf2, Natascha Leijnse1, Janine Erler2, Lene Oddershede1
1University of Copenhagen, The Niels Bohr Institute, Blegdamsvej 17, 2100 Copenhagen Ø, Denmark
2University of Copenhagen, Biotech Research and Innovation Centre (BRIC), Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark
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Cancer cells divide uncontrollably and can be highly invasive into adjacent tissues, thereby posing a large threat to the patient they reside in. Characterizing the dynamics of cancer tissue may lead to a better understanding of the metastatic process, which is one of the main challenges in today’s cancer treatment. Recently, it was reported that a cell division in a two-dimensional sheet of endothelial cells exerts forces on the surrounding cells, resulting in long-range well-ordered hydrodynamic patterns [1]. We investigated the dynamics induced by cell division within a two-dimensional confluent cell layer of human and murine breast cancer cells. Invasive and non-invasive, rounded and elongated, cells from both species were chosen for comparison: 67NR (human, elongated, non-invasive), 4T1 (human, round, invasive), MCF-7 (mouse, round, non-invasive), and MDA-MB-231 (mouse, elongated, invasive) cells. We imaged cell proliferation over timescales of several hours using time-lapse microscopy. Applying particle image velocimetry (PIV), we calculated the resulting monolayer displacements (the velocity field) by correlating the changes in contrast of two sequential images. From the calculated velocity field, the tendency of swirling (the vorticity field), and the tendency of the tissue to expand or contract (the divergence) were extracted. To increase the signal to noise ratio, the velocity fields and all subsequent analyses were averaged over at least 30 data sets. The divergence of all cell lines displayed a local expansion at the center of division, consistent with the daughter cells moving apart after division. The vorticity fields of all cell lines revealed two vortex pairs at the division site, hence, displaying dynamics significantly different from that of endothelial tissue [1]. The vorticities of invasive cancer tissues were significantly stronger than of the non-invasive cancer tissues, regardless of cell shape. Also, aggressive cancer cells within the tissue moved significantly faster than non-aggressive cells, which still moved faster than endothelial cells [1]. Characterization of the dynamics of invasive and non-invasive cancer cell lines is one step in the direction of understanding better the dynamics and mechanisms governing metastasis.
[1]Rossen, N. S.; Tarp, J. M.; Mathiesen, J.; Jensen, M. H.; Oddershede, L. B.Long-Range Ordered Vorticity Patterns in Living Tissue Induced by Cell Division, Nature Communications (2014)
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