6th Annual Symposium
Physics of Cancer
September 7-9, 2015
|PoC - Physics of Cancer - Annual Symposium|
Cancer cell mechanotype: from screening to disease biophysics
Department of Integrative Biology and Physiology, UCLA, 612 Charles E. Young Drive East Box 957246 Los Angeles, CA 90095-7246, USA
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Cell mechanical phenotype or ‘mechanotype’ determines how physical stresses are transduced into the cell, and can also signal a transformation in a cell’s physiological state, such as in malignant transformation. If we could obtain higher throughput measurements of cell mechanotype, this would enable a deeper understanding of the molecular origins of cell mechanotype, as well as screening based on mechanotype. To address these questions, we recently invented a mechanotyping platform that we call Parallel Microfiltration (PMF). I will discuss how we are applying our PMF method to detect cells based on their status in epithelial-to-mesenchymal transition, chemoresistance, as well as the effects of small molecules that have anti-cancer effects. Using our mechanotyping devices, we are also establishing the role of the nuclear structural protein, lamin A, in regulating the mechanical properties of pancreatic cancer cells, and in modulating levels of reactive oxygen species when cells are subject to physical stresses of the tumor microenvironment.