6th Annual Symposium
Physics of Cancer
September 7-9, 2015
|PoC - Physics of Cancer - Annual Symposium|
Dynamical behavior of cancer cells migrating on nano- and micro-patterned substrates
Institut des Nanotechnologies de Lyon, UMR5270 – Université Claude Bernard Lyon 1, Bâtiment Brillouin-203, Rez de Chaussée 8 rue André-Marie Ampère , Campus de la Doua, F-69 622 Villeurbanne France
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Metastasis is a complex process characterized by the detachment of invasive cells from the primary tumor. Metastatic cells migrate through the Extra Cellular Matrix (ECM) to reach the blood circulation, they transmigrate through the endothelium of the blood vessels, they are carried by the blood flow towards remote organs, where they can finally colonize and proliferate to form metastases. Understanding the mechanistic influence of the tumor microenvironment on cancer cell migration and transmigration, represents a major challenge for the development of new cancer clinical strategies based on personalized diagnosis, prognosis and drug screening.
In this study, we investigate the effect of nano- and micro-patterned substrates on the cells morphology and their migratory properties using microfluidic approaches. We first report the migratory behavior of cancer cells on a synthetic ECM in the form of nanoscale model substrates to test their migration potentials. Anisotropically patterned surfaces, obtained by molding oriented nanofibers, are used as a model of the prominent component of the extracellular matrix.
We then study the mechanical mechanisms underlying the transmigration of breast cancer cells. In particular, we have highlighted how physical environment of tumor cells interplay with their mechanical properties to influence their transmigration through pores of varying size.
In both cases, we show that cells tend to show directed migration, hence highlighting the influence of the physical environment of the cell on their dynamic behavior.